Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1560 |
| PDBID: | 5M14 |
| Chains: | C_A |
| Organism: | Escherichia coli K-12, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 1.60 |
| Reference: | 10.7554/eLife.34317 |
| Antibody | |
| Antibody: | L2_G11 synthetic-Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Maltose-binding periplasmic protein |
| Antigen mutation: | No |
| Durg Target: | P0AEX9 |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >5M14_C|Chain C, D|synthetic Nanobody L2_G11 (a-MBP#2)|synthetic construct (32630) GPSQVQLVESGGGSVQAGGSLRLSCAASGQIEHIGYLGWFRQAPGKEREGVAALITYTGHTYYADSVKGRFTVSLDNAKNTVYLQMNSLKPEDTALYYCAAAEWGSQSPLTQWFYRYWGQGTQVTVSA |
Antigen
Chain: A
Mutation: NULL
| >5M14_A|Chain A, B|Maltose-binding periplasmic protein|Escherichia coli K-12 (83333) GPSKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTPGSA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: PRO-1 SER0 GLU29 HIS30 GLY32 TYR33 ARG45 ILE52 TYR54 THR55 HIS57 TYR59 TYR95 GLU100 TRP101 GLY102 SER103 GLN104 SER105 PRO106 LEU107 GLN109 TRP110 TYR112 ARG113 TYR114 TRP115 GLY116 GLN117 A: ASN12 GLY13 ASP14 LYS15 ASP41 LYS42 GLU44 GLU45 GLN49 ASP65 ARG66 GLU111 ASN150 GLN152 GLU153 TYR155 PHE156 ASP207 ASP209 TYR210 SER211 ILE212 GLU214 TRP230 ASN234 MET330 SER337 ALA338 TRP340 TYR341 ARG344 ILE348 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
