Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1557 |
| PDBID: | 5LWF |
| Chains: | D_B |
| Organism: | Bacillus licheniformis, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.56 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | cAb-G10S VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Beta-lactamase |
| Antigen mutation: | No |
| Durg Target: | P00808 |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >5LWF_D|Chain C, D|Camelid heavy-chain antibody variable fragment cAb-G10S|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCAASGRTFSNDHMGWFRKAPGKEREFVAAITPGTEKTYYADSVKGRFAFSRDNAKNTVYLQMNSLKPEDTAVYYCVATPYYRGSYYAASTYTYWGQGTQVTVSSHHHHHH |
Antigen
Chain: B
Mutation: NULL
| >5LWF_B|Chain A, B|Beta-lactamase|Bacillus licheniformis (1402) TEMKDDFAKLEEQFDAKLGIFALDTGTNRTVAYRPDERFAFASTIKALTVGVLLQQKSIEDLNQRITYTRDDLVNYNPITEKHVDTGMTLKELADASLRYSDNAAQNLILKQIGGPESLKKELRKIGDEVTNPERFEPELNEVNPGETQDTSTARALVTSLRAFALEDPGKLPSEKRELLIDWMKRNTTGDALIRAGVPDGWEVADKTGAASYGTRNDIAIIWPPKGDPVVLAVLSSRDKKDAKYDDKLIAEATKVVMKALNMNGKGPHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: LYS42 GLU47 ARG48 LYS60 THR61 TYR62 LYS68 TYR105 ARG106 GLY107 SER108 TYR109 TYR110 ALA111 ALA112 SER113 THR114 B: ARG99 ASP100 ASP101 LEU102 VAL103 ASN104 ASN136 LYS140 PRO145 GLU163 ARG164 PHE165 PRO167 GLU168 GLU171 GLN178 LYS270 ASP271 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
