Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1554 |
| PDBID: | 5LSP |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1038/s41598-017-09460-2 |
| Antibody | |
| Antibody: | 107_A07 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hepatocyte growth factor receptor |
| Antigen mutation: | No |
| Durg Target: | P08581; Q59EB3; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5LSP_H|Chain B[auth H], E[auth S]|107_A07 Fab heavy chain|Homo sapiens (9606) QMQLVQSGAEVKKPGAPVKVSCKVSGYTFTDYYMHWVQQAPGKGLEWMGLVDPEDGETIYAEKFQGRVTITADTSTDTAYMELSSLRSEDTAVYYCATDATTPYWGMMWWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >5LSP_L|Chain C[auth L], F[auth T]|107_A07 Fab light chain|Homo sapiens (9606) ASDIQMIQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGRAPKVLIYDASNLETGVPSRFSGSGSGTEFTLTISNLRPDDFATYYCQQGDSFPLTFGGGTKVEIKRAAAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKLYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >5LSP_A|Chain A, D[auth P]|Hepatocyte growth factor receptor|Homo sapiens (9606) GCRHFQSCSQCLSAPPFVQCGWCHDKCVRSEECLSGTWTQQICLPAIYKVFPNSAPLEGGTRLTICGWDFGFRRNNKFDLKKTRVLLGNESCTLTLSESTMNTLKCTVGPAMNKHFNMSIIISNGHGTTQYSTFSYVDPVITSISPKYGPMAGGTLLTLTGNYLNSGNSRHISIGGKTCTLKSVSNSILECYTPAQTISTEFAVKLKIDLANRETSIFSYREDPIHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR30 ASP31 TYR32 TYR33 LEU50 ASP52 GLU54 ASP55 GLU57 ILE59 ASP99 ALA100 THR101 THR102 PRO103 TYR104 TRP105 L: GLN27 ASP28 ILE29 SER30 TYR32 GLY91 ASP92 SER93 PHE94 A: ARG580 ARG592 ASN593 LYS595 ASP597 LEU598 LYS599 LYS600 ARG602 THR611 LEU612 THR613 LEU614 SER615 GLU616 THR625 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
