Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1553 |
| PDBID: | 5LQB |
| Chains: | HL_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 1.95 |
| Reference: | 10.1126/scitranslmed.aag3187 |
| Antibody | |
| Antibody: | NARA1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-2 |
| Antigen mutation: | Yes |
| Durg Target: | P60568 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5LQB_H|Chain B[auth H]|anti-hIL2 FAB fragment heavy chain|Mus musculus (10090) QVQLQQSGAELVRPGTSVKVSCKASGYAFTNYLIEWVKQRPGQGLEWIGVINPGSGGTNYNEKFKGKATLTADKSSSTAYMQLSSLTSDDSAVYFCARWRGDGYYAYFDVWGAGTTVTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGPT |
Light Chain: L
Mutation: NULL
| >5LQB_L|Chain C[auth L]|anti-hIL2 FAB fragment light chain|Mus musculus (10090) DIVLTQSPASLAVSLGQRATISCKASQSVDYDGDSYMNWYQQKPGQPPKLLIYAASNLESGIPARFSGSGSGTDFTLNIHPVEEEDAATYYCQQSNEDPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: C124S
| >5LQB_A|Chain A|Interleukin-2|Homo sapiens (9606) PTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFSQSIISTLT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR30 ASN31 LEU33 GLU35 TRP47 VAL50 ASN52 SER55 GLY56 GLY57 THR58 ASN59 TRP99 GLY101 ASP102 GLY103 TYR104 TYR105 ALA106 L: TYR31 ASP32 GLY33 ASP34 TYR36 SER95 ASN96 GLU97 ASP98 TYR100 A: GLY47 ASN50 LYS52 ASN53 PRO54 LYS55 THR57 ARG58 THR61 PHE62 LYS63 GLU88 ASN91 LEU92 GLN94 SER95 LYS96 ASN97 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
