Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1546 |
| PDBID: | 5LCV |
| Chains: | HL_A |
| Organism: | Zika virus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.64 |
| Reference: | 10.1038/nature18938 |
| Antibody | |
| Antibody: | EDE2 A11 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Zika virus Polyprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5LCV_H|Chain C[auth H]|BROADLY NEUTRALIZING HUMAN ANTIBODY EDE2 A11|Homo sapiens (9606) EVQLVESGGGLVRPGGSLRLSCAASGFSYSNHWMHWVRQAPGKGLVWVSRINSDGSTRNYADFVKGRFTISRDNAENTLYLEMNSLTADDTAVYYCVRDGVRFYYDSTGYYPDSFFKYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPLEDDDDKAGWSHPQFEKGGGSGGGSGGGSWSHPQFEK |
Light Chain: L
Mutation: NULL
| >5LCV_L|Chain D[auth L]|BROADLY NEUTRALIZING HUMAN ANTIBODY EDE2 A11|Homo sapiens (9606) QSVLTQPVSVSGSPGQSITISCTGTSSNADTYNLVSWYQQRPGKAPKLMIYEGTKRPSGVSNRFSASKSATAASLTISGLQPEDEADYYCCSYATSRTLVFGGGTKLTVVGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >5LCV_A|Chain A, B|Polyprotein|Zika virus (64320) IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGGPFEDDDDKAGWSHPQFEKGGGSGGGSGGGSWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER52 ASP53 SER55 THR56 ARG98 L: SER94 A: MET68 ALA69 SER70 ASP71 SER72 ARG73 CYS74 TYR81 LEU82 ASP83 VAL97 ASP98 ARG99 TRP101 GLY102 ASN103 GLY104 LEU113 LYS251 ARG252 GLN253 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
