Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1545 |
| PDBID: | 5LBS |
| Chains: | IM_B |
| Organism: | Zika virus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.41 |
| Reference: | 10.1038/nature18938 |
| Antibody | |
| Antibody: | EDE1-C8 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | envelope protein E |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >5LBS_I|Chain C[auth H], D[auth I]|BROADLY NEUTRALIZING HUMAN ANTIBODY EDE1 C8|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCSASGFTFSTYSMHWVRQAPGKGLEYVSAITGEGDSAFYADSVKGRFTISRDNSKNTLYFEMNSLRPEDTAVYYCVGGYSNFYYYYTMDVWGQGTTVTVSSGTGGSGGGGSGGGGSGGGA |
Light Chain: M
Mutation: NULL
| >5LBS_M|Chain E[auth L], F[auth M]|BROADLY NEUTRALIZING HUMAN ANTIBODY EDE1 C8|Homo sapiens (9606) SEIVLTQSPATLSLSPGERATLSCRASQSISTFLAWYQHKPGQAPRLLIYDASTRATGVPARFSGSRSGTDFTLTISTLEPEDFAVYYCQQRYNWPPYTFGQGTKVEIKAAADDDDKAGWSHPQFEKGGGSGGGSGGGSWSHPQFEK |
Antigen
Chain: B
Mutation: NULL
| >5LBS_B|Chain A, B|envelope protein E|Zika virus (64320) IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGGPFEDDDDKAGWSHPQFEKGGGSGGGSGGGSWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: TYR96 ASN98 M: SER30 THR31 PHE32 ASP50 SER52 THR53 ARG54 ALA60 GLY64 ARG66 B: ARG2 THR315 LYS316 GLU329 GLN331 THR369 GLU370 LYS373 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
