Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1541 |
| PDBID: | 5L0Q |
| Chains: | FE_D |
| Organism: | Bos taurus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.76 |
| Reference: | 10.1084/jem.20151095 |
| Antibody | |
| Antibody: | 8C7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Disintegrin and metalloproteinase domain-containing protein 10 |
| Antigen mutation: | No |
| Durg Target: | O14672 |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >5L0Q_F|Chain C, F|mAb 8C7 heavy chain|Homo sapiens (9606) QVQLEESGAELARPGSSVKLSCKASGYTFTNYWLQWVKQRTGQGLEWIGAIYPRDGDAKYSQKFKDKASLTVNESSSTAYMHLSALASEDSAVYYCARANYGLYYAMDRWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPWPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: E
Mutation: NULL
| >5L0Q_E|Chain B, E|mAb 8C7 light chain|Homo sapiens (9606) DIFLTQSPANMSVSPGERVSFSCRASQNIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFILSINTVESEDIAVYFCQQSNRWPFTFGSGTKLEVIRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: D
Mutation: NULL
| >5L0Q_D|Chain A, D|Disintegrin and metalloproteinase domain-containing protein 10|Bos taurus (9913) PVGLASGQPICGNGMVEQGEECDCGYSDQCKDECCYDANQPEGKKCKLKPGKQCSPSQGPCCTAHCAFKSKTEKCRDDSDCAKEGICNGITALCPASDPKPNFTDCNRHTQVCINGQCAGSICEKHGLEECTCASSDGKDDKELCHVCCMKKMEPSTCASTGSVQWNKYFLGRTITLQPGSPCNDFRGYCDVFMRCRGSASGL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: SER65 GLY66 ILE72 SER91 ASN92 ARG93 TRP94 PHE96 F: ASN31 TYR32 TRP33 GLN35 TRP47 ALA50 TYR52 ASP55 ASP57 LYS59 ALA99 LEU103 TYR104 TYR105 D: GLY452 ARG557 ASP590 PRO628 GLY629 TYR638 CYS639 ASP640 VAL641 PHE642 MET643 ARG644 ARG646 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
