Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 154 |
| PDBID: | 1RJL |
| Chains: | BA_C |
| Organism: | Mus musculus, Borreliella burgdorferi |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1074/jbc.M412842200 |
| Antibody | |
| Antibody: | H6831 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Outer surface protein B |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >1RJL_B|Chain B|Fab H6831 H-chain|Mus musculus (10090) QVQLQQPGSVLVRPGASVKLSCKASGFTFTSSWMHWAKQRPGQGLEWIGEIHPNSGNTHYNEKFKGKATLTVDTSSSTAYVDLSSLTSEDSAVYYCARMRYGDYYAMDNWGQGTSVTVSSAKTTAPPVYPLAPVCGDTTGSSVTLGCLVKGYFPESVTLLWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRG |
Light Chain: A
Mutation: NULL
| >1RJL_A|Chain A|Fab H6831 L-chain|Mus musculus (10090) DIQMNQSPSSLSASLGDTITITCHASQNINVWLNWFQQKPGSIPKLLIYMASNLHTGVPSRFSGSGSGTGFTLTISSLQPEDIATYYCQQGQSFPLTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN |
Antigen
Chain: C
Mutation: NULL
| >1RJL_C|Chain C|Outer surface protein B|Borrelia burgdorferi (139) TVEIKEGTVTLKREIEKDGKVKVFLNDTAGSNKKTGKWEDSTSTLTISADSKKTKDLVFLTDGTITVQQYNTAGTSLEGSASEIKNLSELKNALK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: TRP32 GLY91 GLN92 SER93 PHE94 B: THR28 THR30 SER31 TRP33 GLU50 HIS52 ASN54 HIS59 TYR101 GLY102 TYR105 C: GLY231 SER232 ASN233 ALA250 ASP251 SER252 LYS253 LYS254 ASN272 THR273 ALA274 GLY275 THR276 SER277 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
