Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1535 |
| PDBID: | 5KW9 |
| Chains: | HL_A |
| Organism: | Norwalk virus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.30 |
| Reference: | 10.1073/pnas.1609990113 |
| Antibody | |
| Antibody: | 5KW9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Capsid protein VP1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5KW9_H|Chain B[auth H]|IgA(VH)-IgG(CH) heavy chain Fab fragment|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCRTSGDTFNTHAISWVRQAPGQGLEWMGGIIPIFATTNYANKFQGTVTISADESTSTAYLEVRSLRSEDTAVYYCASNRANRADDYDYYFDYWGQGTLVTVSSASFKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >5KW9_L|Chain C[auth L]|IgA Light chain|Homo sapiens (9606) EIVMSQSPDSLAVSLGERATINCKSSQSVLYKSDKKNYLAWYQQKSGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSIPRTFGQGTKVDIKRTAAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHEGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >5KW9_A|Chain A|Capsid protein VP1|Norwalk virus (11983) EQKTRPFTLPNLPLSSLSNSRAPLPISSMGISPDNVQSVQFQNGRCTLDGRLVGTTPVSLSHVAKIRGTSNGTVINLTELDGTPFHPFEGPAPIGFPDLGGCDWHINMTQFGHSSQTQYDVDTTPDTFVPHLGSIQANGIGSGNYVGVLSWISPPSHPSGSQVDLWKIPNYGSSITEATHLAPSVYPPGFGEVLVFFMSKMPGPGAYNLPCLLPQEYISHLASEQAPTVGEAALLHYVDPDTGRNLGEFKAYPDGFLTCVPNGASSGPQQLPINGVFVFVSWVSRFYQLKPVGTA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ALA104 ASP105 ASP106 TYR107 ASP108 L: GLN27 SER28 LEU30 TYR31 LYS32 SER33 LYS35 TYR38 GLY74 THR75 TYR98 SER99 ILE100 A: ASP346 THR347 THR348 PRO349 ASP350 THR351 PHE352 GLN360 ASN362 SER380 HIS381 PRO382 SER383 GLY384 SER385 ASN394 GLY396 SER397 SER398 ILE399 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
