Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1532 |
| PDBID: | 5KVD |
| Chains: | HL_E |
| Organism: | Zika virus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 1.65 |
| Reference: | 10.1016/j.cell.2016.07.020 |
| Antibody | |
| Antibody: | ZV-2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Zika Envelope DIII |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5KVD_H|Chain C[auth H]|ZV-2 Antibody Fab Heavy Chain|Mus musculus (10090) EVQLQESGAELMKPGASVKLSCKTSGYTFIGYWIEWLKQRPGHGLEWVGEIFPGSGRTKYNEKFKGRATFTADTSSNMAYMQLSSLTTEDSAIYYCARYYYGSYYALDYWGQGTSVTVSSAKTTAPSVYPLAPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIEPRVP |
Light Chain: L
Mutation: NULL
| >5KVD_L|Chain B[auth L]|ZV-2 Antibody Fab Light Chain|Mus musculus (10090) DIVMTQSPSSLSVSAGEKVTLSCKSSQSLLHSGNQKNYLAWYQQKPGQAPKLLIYGASTRESGVPDRFTGSGSGTDFTLTISSVQAEDLAVYYCQNDHSYPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: E
Mutation: NULL
| >5KVD_E|Chain A[auth E]|Zika Envelope DIII|Zika virus (64320) MRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR32 TYR49 THR53 GLU55 SER56 H: VAL2 SER25 GLY26 TYR27 THR28 PHE29 ILE30 GLY31 TYR32 GLY53 ASN76 ARG94 TYR96 TYR97 GLY98 SER99 ASP101 TYR102 E: ARG299 LYS301 THR315 LYS316 ILE317 ALA319 GLU320 THR321 LEU322 HIS323 GLY324 THR327 GLU329 THR360 ALA361 ASN362 VAL364 ILE365 THR366 GLU367 ASN371 SER372 LYS373 MET374 MET375 GLU377 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
