Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1530 |
| PDBID: | 5KU0 |
| Chains: | 7_3 |
| Organism: | Human poliovirus 1 Mahoney, Camelus dromedarius |
| Method: | EM |
| Resolution (Å): | 5.30 |
| Reference: | 10.1128/JVI.01443-16 |
| Antibody | |
| Antibody: | 17B VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | expanded poliovirus VP3 |
| Antigen mutation: | No |
| Durg Target: | P03300 |
Sequence information
Antibody
Chain: 7
Mutation: NULL
| >5KU0_7|Chain D[auth 7]|VHH 17B|Camelus dromedarius (9838) QVQLQESGGGLVQPGGSLTLSCAASGYAVSRYSMGWFRQAPGKENEGVAAIDSSGVGTTYADSVKGRFTISRDNAKDTVYLRMNSLKPEDTAIYYCASGFGLSLSRYTYAYWGQGTQVTVSSHH |
Antigen
Chain: 3
Mutation: NULL
| >5KU0_3|Chain C[auth 3]|VP3|Poliovirus type 1 (strain Mahoney) (12081) GLPVMNTPGSNQYLTADNFQSPCALPEFDVTPPIDIPGEVKNMMELAEIDTMIPFDLSATKKNTMEMYRVRLSDKPHTDDPILCLSLSPASDPRLSHTMLGEILNYYTHWAGSLKFTFLFCGSMMATGKLLVSYAPPGADPPKKRKEAMLGTHVIWDIGLQSSCTMVVPWISNTTYRQTIDDSFTEGGYISVFYQTRIVVPLSTPREMDILGFVSACNDFSVRLLRDTTHI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| 7: GLN1 VAL2 GLY26 TYR27 ARG31 TYR32 GLN39 GLU44 ASN45 ASP52 SER54 THR59 ASP62 TYR95 PHE100 GLY101 LEU102 SER103 LEU104 SER105 ARG106 TYR107 THR108 TYR109 ALA110 TYR111 TRP112 GLY113 GLN114 3: PHE55 ASP56 LEU57 SER58 ALA59 THR60 LYS61 LYS62 ARG69 VAL70 ARG71 LYS75 PRO76 THR78 ASP80 PRO81 ILE82 LEU83 CYS84 SER86 SER91 ASP92 PRO93 ARG94 ASP140 PRO141 PRO142 LYS143 LYS144 ASP181 SER183 PHE184 GLU186 TYR189 GLU207 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
