Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1528 |
| PDBID: | 5KTE |
| Chains: | HL_A |
| Organism: | Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.94 |
| Reference: | 10.1016/j.str.2016.09.017 |
| Antibody | |
| Antibody: | 5KTE Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Divalent metal cation transporter MntH |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5KTE_H|Chain B[auth H]|Fab Heavy Chain|Mus musculus (10090) QVQLTESGPGLVAPSQSLSITCTVSGFSLTSYGVHWVRQPPGKGLEWLVVIWSDGSTTYNSALKSRLSISKDNSKSQVFLKMNSLQTDDTAMYYCAREPPYGYWGQGTTLTVSSAKTTPPSVYPLAPGCASTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEP |
Light Chain: L
Mutation: NULL
| >5KTE_L|Chain C[auth L]|Fab Light Chain|Mus musculus (10090) DIELTQSPATLSVTPGDSVSLSCRASQSISNNLHWYQQKSHESPRLLIKYVSQSSSGIPSRFSGSGSGTDFTLSINSVETEDFGMYFCQQSNSWPRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: A
Mutation: NULL
| >5KTE_A|Chain A|Divalent metal cation transporter MntH|Deinococcus radiodurans (243230) MHHHHHHHHATLRGTAGPRGVRRILPFLGPAVIASIAYMDPGNFATNIEGGARYGYSLLWVILAANLMAMVIQNLSANLGIASGRNLPELIRERWPRPLVWFYWIQAELVAMATDLAEFLGAALAIQLLTGLPMFWGAVVTGVVTFWLLNLHHRGTRPLELAVGAFVLMIGVAYLVQVVLARPDLAAVGAGFVPRLQGPGSAYLAVGIIGATVMPHVIYLHSALTQGRIQTDTTYYYRRLVRLNRVDVIAAMGLAGLINMSMLAVAAATFHGKNVENAGDLTTAYQTLTPLLGPAASVLFAVALLASGLSSSAVGTMAGDVIMQGFMGFHIPLWLRRLITMLPAFIVILLGMDPSSVLILSQVILCFGVPFALVPLLLFTAHHDVMGALVTRRSFTVIGWVIAVIIIALNGYLLWELLGG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER31 TYR32 GLY33 HIS35 VAL50 TRP52 ASP54 SER56 GLU98 PRO99 PRO100 TYR101 L: ASP1 SER30 ASN32 HIS34 LYS49 TYR50 SER91 ASN92 SER93 TRP94 PRO95 ARG96 A: ALA68 ARG69 TYR70 TYR72 ALA197 ARG198 ALA202 LEU212 ALA283 ALA284 HIS287 GLY288 LYS289 ASN290 VAL291 LEU304 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
