Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1523 |
| PDBID: | 5KOV |
| Chains: | W_S |
| Organism: | Human astrovirus 2, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.25 |
| Reference: | 10.1128/JVI.01859-16 |
| Antibody | |
| Antibody: | PL-2 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Capsid polyprotein VP90 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: W
Mutation: NULL
| >5KOV_W|Chain B[auth C], C[auth E], F[auth I], G[auth K], J[auth O], K[auth Q], N[auth U], O[auth W]|PL-2 scFv chain|Mus musculus (10090) RSDVQLKQSGPGLVQPSQSLSITCTVSGFSLIDYGVHWVRQSPGKGLEWLGVIWTGGSTDYNAAFISRLTISKDNSKSQVFFKMNSLQANDTGIYYCGRPYYGNVMDYWGQGTSVTVSSGTGGSGGGGSGGGGSGGGASDIQMTQTTSSLSASLGDRVTISCRASQDISNYLNWYQQKPDGTVKLLIYYTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTFPPTFGGGTKLEIKRLVPR |
Antigen
Chain: S
Mutation: NULL
| >5KOV_S|Chain A, D[auth B], E[auth G], H, I[auth M], L[auth N], M[auth S], P[auth T]|Capsid polyprotein VP90|Human astrovirus-2 (12701) MGGELRVLLTVGSIMSPNSADRQVWLNKTLTAPGTNSNDNLVKIAHDLGHYLIMQGFMHIKTVEWYTPDFQPSRDPTPIAGMSVMVNITKKADVYFMKQFKNSYTNNRHQITSIFLIKPLADFKVQCYMSYFKRESHDNDGVANLTVRSMTSPETIRFQVGEWYLLTSTTLKENNLPEGWVWDRVELKSDTPYYADQALTYFITPPPVDSQILFEGNTAAAELALVPR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| W: ILE30 ASP31 TYR32 HIS35 TRP47 VAL50 TRP52 THR53 GLY54 ASP58 TYR99 TYR100 GLY101 ASN102 SER1030 ASN1031 TYR1032 TYR1049 TYR1050 ARG1053 GLY1091 ASN1092 THR1093 PHE1094 PRO1096 S: ALA458 PRO459 GLY460 THR461 ASN462 SER463 ASP465 ASN466 ASN513 ILE514 THR515 LYS517 LYS550 GLN552 TYR554 MET576 THR577 SER578 PRO579 GLU580 THR581 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
