Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1516 |
| PDBID: | 5KEN |
| Chains: | JI_E |
| Organism: | Ebola virus - Mayinga, Zaire, 1976, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 4.30 |
| Reference: | 10.1038/nmicrobiol.2016.128 |
| Antibody | |
| Antibody: | c13C6 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola surface glycoprotein, GP1 |
| Antigen mutation: | No |
| Durg Target: | Q05320 |
Sequence information
Antibody
Heavy Chain: J
Mutation: NULL
| >5KEN_J|Chain J, P[auth Q]|c13C6 variable Fab domain heavy chain|Homo sapiens (9606) DVKLLESGGGLVQPGGSLKLSCAASGFSLSTSGVGVGWFRQPSGKGLEWLALIWWDDDKYYNPSLKSQLSISKDFSRNQVFLKISNVDIADTATYYCARRDPFGYDNAMGYWGQGTSVTVS |
Light Chain: I
Mutation: NULL
| >5KEN_I|Chain I, O[auth P]|c13C6 variable Fab domain light chain|Homo sapiens (9606) DIVMTQSPLSLSTSVGDRVSLTCKASQNVGTAVAWYQQKPGQSPKLLIYSASNRYTGVPDRFTGSGSGTDFTLTISNMQSEDLADYFCQQYSSYPLTFGAGTKLELR |
Antigen
Chain: E
Mutation: NULL
| >5KEN_E|Chain A, E, K|Ebola surface glycoprotein, GP1|Zaire ebolavirus (128952) IPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWKVNPEIDTTIGEWAFWETKKNLTRKIRSEELSF |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: VAL29 GLY30 ALA32 TYR91 SER92 SER93 TYR94 LEU96 J: SER30 THR31 SER32 GLY33 LEU50 TRP52 TRP53 ASP54 ASP55 ASP56 TYR58 LYS71 ASP72 PHE73 SER74 ARG95 PRO97 PHE98 GLY99 E: PRO116 ASP117 GLY143 THR144 GLN221 ALA222 THR223 GLY224 LEU233 GLU235 THR240 ASN268 THR269 THR270 GLY271 LYS272 LEU273 ILE274 GLU305 SER307 PHE308 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
