Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1512 |
| PDBID: | 5KEN |
| Chains: | CD_B |
| Organism: | Ebola virus - Mayinga, Zaire, 1976, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 4.30 |
| Reference: | 10.1038/nmicrobiol.2016.128 |
| Antibody | |
| Antibody: | c4G7 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola surface glycoprotein, GP2 |
| Antigen mutation: | No |
| Durg Target: | Q05320 |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >5KEN_C|Chain C, G, M[auth N]|c4G7 variable Fab domain heavy chain|Homo sapiens (9606) EVQLQESGPELEMPGASVKISCKASGSSFTGFSMNWVKQSNGKSLEWIGNIDTYYGGTTYNQKFKGKATLTVDKSSSTAYMQLKSLTSEDSAVYYCARSAYYGSTFAYWGQGTLVTVS |
Light Chain: D
Mutation: NULL
| >5KEN_D|Chain D, H, N[auth O]|c4G7 variable Fab domain light chain|Homo sapiens (9606) DIQMTQSPASLSASVGETVTITCRASENIYSYLAWYQQKQGKSPQLLVYNAKTLIEGVPSRFSGSGSGTQFSLKINSLQPEDFGSYFCQHHFGTPFTFGSGTELEIK |
Antigen
Chain: B
Mutation: NULL
| >5KEN_B|Chain B, F, L[auth M]|Ebola surface glycoprotein, GP2|Zaire ebolavirus (strain Mayinga-76) (128952) AIVNAQPKCNPNLHYWTTQDEGAAIGLAWIPYFGPAAEGIYTEGLMHNQDGLICGLRQLANETTQALQLFLRATTELRTFSILNRKAIDFLLQRWGGTCHILGPDCCIEPHDW |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: GLY31 PHE32 SER33 ASN50 TYR53 TYR54 THR58 ARG94 TYR97 TYR98 GLY99 D: TYR30 TYR32 TYR49 ASN50 THR53 GLU56 HIS91 PHE92 GLY93 THR94 B: ALA503 ILE504 VAL505 ASN506 ALA507 GLN508 PRO509 LYS510 CYS511 ASN512 PRO513 ASN514 HIS516 GLU545 GLY546 LEU547 HIS549 ASN550 GLN551 ASP552 GLY553 CYS556 GLY557 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
