Details

Structure visualisation

Display styles :





Display color :





Entry information

Complex
AACDB_ID: 1511
PDBID: 5KEM
Chains: GH_F
Organism: Homo sapiens, Ebola virus - Mayinga, Zaire, 1976
Method: EM
Resolution (Å): 5.50
Reference: 10.1038/nmicrobiol.2016.128
Antibody
Antibody: BDBV91 Fab
Antibody mutation: No
INN (Clinical Trial):
Antigen
Antigen: Ebola secreted glycoprotein
Antigen mutation: No
Durg Target: Q05320; Q05320;

Sequence information

Antibody

Heavy Chain: G
Mutation: NULL

>5KEM_G|Chain B, G|BDBV91 variable Fab domain heavy chain|Homo sapiens (9606)
QVQLVQSGAELKPPGASVKVSCKPSGYTFTDYYIHWVRQAPGQGLEWMGWINPKSGETHYAQKFRGWVTLTRDTSISTTYMDLTRLKSDDTAVYFCARGDLETTIFFYNAVDVWGQGTLVT

Light Chain: H
Mutation: NULL

>5KEM_H|Chain A[auth C], F[auth H]|BDBV91 variable Fab domain light chain|Homo sapiens (9606)
DIQMTQSPSSLSASVGDRVTITCRATESIGIYLNWYQRKPGKAPNLLIFATSSLQSGVPSRFSGSGSGTEFTLTISSLQPEDFATYFCQQGFSSPFSFGQGTRLEIK

Antigen

Chain: F
Mutation: NULL

>5KEM_F|Chain E[auth A], J[auth F]|Ebola secreted glycoprotein|Zaire ebolavirus (128952)
CRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWKVNPEIDTT

Interaction

1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.

Interacting residues (ΔSASA based)

H: SER28 ILE29 GLY30 ILE31 TYR32 PHE92 SER93 SER94 PHE96

G: TYR33 TRP50 HIS58 TYR59 LEU97 GLU98 THR99

F: LYS84 ARG89 SER90 GLY91 VAL92 PRO93 LYS95 VAL96 VAL97 PHE153 HIS154 LYS155 GLU156 GLY157 PHE159 THR168 THR227

2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.

Interacting residues (Atom distance based)

Download

Download sequences
Download structure
Download interacting residues (ΔSASA based)
Download interacting residues (Distance based)