Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1510 |
| PDBID: | 5KEM |
| Chains: | BC_A |
| Organism: | Homo sapiens, Ebola virus - Mayinga, Zaire, 1976 |
| Method: | EM |
| Resolution (Å): | 5.50 |
| Reference: | 10.1038/nmicrobiol.2016.128 |
| Antibody | |
| Antibody: | BDBV91 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola secreted glycoprotein |
| Antigen mutation: | No |
| Durg Target: | Q05320; Q05320; |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >5KEM_B|Chain B, G|BDBV91 variable Fab domain heavy chain|Homo sapiens (9606) QVQLVQSGAELKPPGASVKVSCKPSGYTFTDYYIHWVRQAPGQGLEWMGWINPKSGETHYAQKFRGWVTLTRDTSISTTYMDLTRLKSDDTAVYFCARGDLETTIFFYNAVDVWGQGTLVT |
Light Chain: C
Mutation: NULL
| >5KEM_C|Chain A[auth C], F[auth H]|BDBV91 variable Fab domain light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRATESIGIYLNWYQRKPGKAPNLLIFATSSLQSGVPSRFSGSGSGTEFTLTISSLQPEDFATYFCQQGFSSPFSFGQGTRLEIK |
Antigen
Chain: A
Mutation: NULL
| >5KEM_A|Chain E[auth A], J[auth F]|Ebola secreted glycoprotein|Zaire ebolavirus (128952) CRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWKVNPEIDTT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: SER28 ILE29 GLY30 ILE31 TYR32 PHE92 SER93 SER94 PHE96 B: TYR33 TRP50 HIS58 TYR59 LEU97 GLU98 THR99 A: LYS84 ARG89 SER90 GLY91 VAL92 PRO93 LYS95 VAL96 VAL97 PHE153 HIS154 LYS155 GLU156 GLY157 PHE159 THR168 THR227 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
