Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1504 |
| PDBID: | 5KEL |
| Chains: | PT_G |
| Organism: | Ebola virus - Mayinga, Zaire, 1976, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 4.30 |
| Reference: | 10.1038/nmicrobiol.2016.128 |
| Antibody | |
| Antibody: | c2G4 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola surface glycoprotein, GP2 |
| Antigen mutation: | No |
| Durg Target: | Q05320 |
Sequence information
Antibody
Heavy Chain: P
Mutation: NULL
| >5KEL_P|Chain C[auth H], I[auth P], O[auth Q]|c2G4 variable Fab domain heavy chain|Homo sapiens (9606) EVQLQESGGGLMQPGGSMKLSCVASGFTFSNYWMNWVRQSPEKGLEWVAEIRLKSNNYATHYAESVKGRFTISRDDSKRSVYLQMNTLRAEDTGIYYCTRGNGNYRAMDYWGQGTSVTVS |
Light Chain: T
Mutation: NULL
| >5KEL_T|Chain D[auth L], J[auth T], P[auth U]|c2G4 variable Fab domain light chain|Homo sapiens (9606) DIQMTQSPASLSVSVGETVSITCRASENIYSSLAWYQQKQGKSPQLLVYSATILADGVPSRFSGSGSGTQYSLKINSLQSEDFGTYYCQHFWGTPYTFGGGTKLEIK |
Antigen
Chain: G
Mutation: NULL
| >5KEL_G|Chain B, H[auth G], N[auth I]|Ebola surface glycoprotein, GP2|Zaire ebolavirus (128952) EAIVNAQPKCNPNLHYWTTQDEGAAIGLAWIPYFGPAAEGIYTEGLMHNQDGLICGLRQLANETTQALQLFLRATTELRTFSILNRKAIDFLLQRWGGTCHILGPDCCIEPHDWTKNITDKIDQIIHDFVDKTLPDLEVDDDD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| P: PHE27 ASN31 TRP33 GLU50 ASN53 ALA56 HIS58 ASN96 GLY97 ASN98 TYR99 T: ILE29 TYR30 SER31 SER32 TYR49 SER50 ALA51 THR52 ILE53 ASP56 TRP92 GLY93 THR94 G: ALA503 ILE504 VAL505 ASN506 ALA507 GLN508 LYS510 CYS511 ASN512 PRO513 ASN514 HIS549 ASN550 GLN551 ASP552 GLY553 CYS556 GLY557 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
