Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1499 |
| PDBID: | 5KAQ |
| Chains: | HL_B |
| Organism: | Influenza A virus (A/Hong Kong/1-4-MA21-1/1968(H3N2)), Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.51 |
| Reference: | 10.1016/j.cell.2016.06.043 |
| Antibody | |
| Antibody: | 31.A.83 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H3N2 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | P03437 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5KAQ_H|Chain C[auth F], F[auth H], H[auth Q]|ANTIBODY 31.A.83 FAB HEAVY CHAIN|Homo sapiens (9606) EVQLLESGGGLVQPGESLRVSCAASGFTFRDSALSWVRQAPGKGLEWVSAISGNGGATYYADTVKGRFTISRDNSQTTLYLQMNSLRADDTATYYCAKDESPPIYNLMPGYYSTYYYMDVWGKGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: L
Mutation: NULL
| >5KAQ_L|Chain D[auth G], G[auth L], I[auth R]|ANTIBODY 31.A.83 FAB LIGHT CHAIN|Homo sapiens (9606) EIVMTQSPVTLSVSPGETATLSCRASQSVRSNLAWYQQKPGQAPRLLIYGASTRATDIPARFSGSGSGTEFTLSISSLQSDDFAVYYCQQYNHWLRTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >5KAQ_B|Chain A, B, E[auth C]|Hemagglutinin|Influenza A virus (A/Hong Kong/1-4-MA21-1/1968(H3N2)) (506380) QDLPGNDNSTATLCLGHHAVPNGTLVKTITDDQIEVTNATELVQSSSTGKICNNPHRILDGIDCTLIDALLGDPHCDVFQNETWDLFVERSKAFSNCYPYDVPDYASLRSLVASSGTLEFITEGFTWTGVTQNGGSNACKRGPGSGFFSRLNWLTKSGSTYPVLNVTMPNNDNFDKLYIWGVHHPSTNQEQTSLYVQASGRVTVSTRRSQQTIIPNIESRPWVRGLSSRISIYWTIVKPGDVLVINSNGNLIAPRGYFKMRTGKSSIMRSDAPIDTCISECITPNGSIPNDKPFQNVNKITYGACPKYVKQNTLKLATGMRNVPEKQTRGLFGAIAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEKFHQIEKEFSEVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTRRQLRENAEDMGNGCFKIYHKCDNACIESIRNGTYDHDVYRDEALNNRFQIKGVSGRLVPR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ASN54 GLY55 GLY56 TYR59 ILE104 TYR105 ASN106 LEU107 MET108 TYR111 TYR112 SER113 THR114 TYR115 L: SER28 ARG30 SER31 SER52 ARG54 SER67 TYR91 B: HIS18 ASN38 THR40 LEU42 ILE278 GLU280 THR318 ILE347 ASP348 GLY349 TRP350 LEU367 THR370 GLN371 ILE374 ASP375 ILE377 ASN378 GLY379 LEU381 ASN382 ILE385 GLU386 LYS387 THR388 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
