Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1488 |
| PDBID: | 5K9O |
| Chains: | HL_I |
| Organism: | Homo sapiens, Influenza A virus (A/California/04/2009(H1N1)) |
| Method: | XRD |
| Resolution (Å): | 3.39 |
| Reference: | 10.1016/j.cell.2016.06.043 |
| Antibody | |
| Antibody: | 31.b.09 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H1N1 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5K9O_H|Chain A, D[auth H]|31.b.09 Heavy Fv|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCKASGYSFSSYGISWVRQAPGQGLEWMGWISAYNGNTNYAQKLQGRVTMTTDTSTSTAYMELRSLRSDDTAVFYCARDRPHILTGFDFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: L
Mutation: NULL
| >5K9O_L|Chain B, F[auth L]|31.b.09 Light Fv|Homo sapiens (9606) DVVMTQSPVSLPVTLGQPASISCRSSQGLVYIDGNTYLNWFQQRPGQSPRRLIYNVFTRDSGVPDRFSGSGSGTDFTLKITTVEAEDVGVYYCMQGTHWPYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLRSPVTKSFNRGEC |
Antigen
Chain: I
Mutation: NULL
| >5K9O_I|Chain C[auth F], E[auth I]|Hemagglutinin|Influenza A virus (strain swl A/California/04/2009 H1N1) (641501) DTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETPSSDNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVTAACPHAGAKSFYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPSTSADQQSLYQNADTYVFVGSSRYSKKFKPEIAIRPKVRDQEGRMNYYWTLVEPGDKITFEATGNLVVPRYAFAMERNAGSGIIISDTPVHDCNTTCQTPKGAINTSLPFQNIHPITIGKCPKYVKSTKLRLATGLRNIPSIQSRGLFGAIAGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDEITNKVNSVIEKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENERTLDYHDSNVKNLYEKVRSQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGTYDYPKYSEEAKLNREEIDGVSG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: TYR54 ASN55 ASN57 ASN59 HIS102 ILE103 LEU104 L: PRO18 GLN27 GLY28 VAL30 TYR31 ILE32 ASP33 GLY34 PHE57 SER72 GLY73 ASP75 THR97 HIS98 TRP99 I: HIS18 ASP24 HIS38 SER39 VAL40 ASN289 THR290 SER291 LYS304 THR318 GLY345 VAL347 ASP348 GLY349 TRP350 ALA365 LEU367 THR370 GLN371 ILE374 ASP375 ILE377 THR378 ASN379 VAL381 ASN382 ILE385 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
