Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1486 |
| PDBID: | 5K9K |
| Chains: | HL_F |
| Organism: | Homo sapiens, Influenza A virus (A/Hong Kong/1/1968(H3N2)) |
| Method: | XRD |
| Resolution (Å): | 2.97 |
| Reference: | 10.1016/j.cell.2016.06.043 |
| Antibody | |
| Antibody: | 56.a.09 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H3N2 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | P03437 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5K9K_H|Chain A, D[auth H]|56.a.09 Heavy chain|Homo sapiens (9606) QVQLQQSGPGLVKPSQTLSLTCVISGDTVSSNRAAWNWIRQSPSRGLEWLGRTYYRSKWYTDYAVSVKSRITITPDTSKNQFSLQMKSVTPEDTAVYYCARGSAMIFGIVIILESWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >5K9K_L|Chain B, E[auth L]|56.a.09 Light chain|Homo sapiens (9606) EIVLTQSPGTLSLSPGERATLSCRASQSVASSYLAWYQQKPGQAPRLLIYGASSRATGVPDRFSGSGSGTDFILTISRLEPEDFAVYYCQQYDGSQYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: F
Mutation: NULL
| >5K9K_F|Chain C[auth F], F[auth I]|Hemagglutinin|Influenza A virus (strain A/Hong Kong/1/1968 H3N2) (506350) QDLPGNDNSTATLCLGHHAVPNGTLVKTITDDQIEVTNATELVQSSSTGKICNNPHRILDGIDCTLIDALLGDPHCDVFQNETWDLFVERSKAFSNCYPYDVPDYASLRSLVASSGTLEFITEGFTWTGVTQNGGSNACKRGPGSGFFSRLNWLTKSGSTYPVLNVTMPNNDNFDKLYIWGVHHPSTNQEQTSLYVQASGRVTVSTRRSQQTIIPNIESRPWVRGLSSRISIYWTIVKPGDVLVINSNGNLIAPRGYFKMRTGKSSIMRSDAPIDTCISECITPNGSIPNDKPFQNVNKITYGACPKYVKQNTLKLATGMRNVPEKQTRGLFGAIAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEKFHQIEKEFSEVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTRRQLRENAEDMGNGCFKIYHKCDNACIESIRNGTYDHDVYRDEALNNRFQIK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ASN32 ARG50 ARG52 SER53 LYS54 TYR56 ASP58 LYS64 ILE99 L: GLU1 SER27 ALA29 SER30 SER31 TYR32 TYR91 ASP92 GLY93 SER94 F: HIS18 VAL20 ASN38 THR318 GLU325 GLU344 GLY345 ILE347 ASP348 GLY349 TRP350 ARG354 GLN363 ALA364 ALA365 LEU367 LYS368 THR370 GLN371 ILE374 ASP375 ILE377 ASN378 ASN475 GLU479 ARG482 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
