Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1483 |
| PDBID: | 5JZ7 |
| Chains: | CD_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.40 |
| Reference: | 10.1038/srep38644 |
| Antibody | |
| Antibody: | MEDI578 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Beta-nerve growth factor |
| Antigen mutation: | No |
| Durg Target: | P01138 |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >5JZ7_C|Chain C, G, H, J|MEDI578 scFv, heavy chain|Homo sapiens (9606) MAQVQLVQSGAEVKKPGSSVKVSCKASGGTFSTYGISWVRQAPGQGLEWMGGIIPIFDTGNSAQSFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARSSRIYDLNPSLTAYYDMDVWGQGTMVTVSS |
Light Chain: D
Mutation: NULL
| >5JZ7_D|Chain D, I, K, L|MEDI578 scFv, light chain|Homo sapiens (9606) SAQSVLTQPPSVSAAPGQKVTISCSGSSSNIGNNYVSWYQQLPGTAPKLLIYDNNKRPSGIPDRFSGSKSGTSATLGITGLQTGDEADYYCGTWDSSLSAWVFGGGTKLTVLGAAAENLYFQ |
Antigen
Chain: A
Mutation: NULL
| >5JZ7_A|Chain A, B, E, F|Beta-nerve growth factor|Homo sapiens (9606) FHRGEFSVCDSVSVWVGDKTTATDIKGKEVMVLGEVNINNSVFKQYFFETKCRDPNPVDSGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTACVCVLSRKA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: THR28 THR31 ILE53 PHE54 SER96 ARG97 ILE98 TYR99 D: GLY29 ASN30 ASN31 TYR32 SER34 ASP50 ASN52 LYS53 LYS66 GLY68 TRP91 SER93 SER94 TRP96 A: HIS8 ARG9 GLY10 GLU11 PHE12 SER13 ASP16 SER17 VAL18 SER19 VAL20 TRP21 LYS50 TYR52 PHE54 THR56 LYS57 ARG59 ASP60 PRO63 VAL64 ASP65 ARG69 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
