Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1465 |
| PDBID: | 5JQH |
| Chains: | C_A |
| Organism: | Tequatrovirus T4, Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.20 |
| Reference: | 10.1038/nature18636 |
| Antibody | |
| Antibody: | Nb60 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Beta-2 adrenergic receptor |
| Antigen mutation: | No |
| Durg Target: | P00720 |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >5JQH_C|Chain C, D|Nanobody60, Nb60|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCAASGSIFSLNDMGWYRQAPGKLRELVAAITSGGSTKYADSVKGRFTISRDNAKNTVYLQMNSLKAEDTAVYYCNAKVAGTFSIYDYWGQGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >5JQH_A|Chain A, B|Endolysin,Beta-2 adrenergic receptor|Enterobacteria phage T4 (10665) DYKDDDDAENLYFQGNIFEMLRIDEGLRLKIYKDTEGYYTIGIGHLLTKSPSLNAAKSELDKAIGRNTNGVITKDEAEKLFNQDVDAAVRGILRNAKLKPVYDSLDAVRRAALINMVFQMGETGVAGFTNSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRVITTFRTGTWDAYAADEVWVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHILTKTWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQEAINCYAEETCCDFFTNQAYAIASSIVSFYVPLVIMVFVYSRVFQEAKRQLQKIDIDKFALKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition C: GLN1 VAL2 GLY26 SER27 ILE28 LEU31 ASN32 SER53 VAL99 ALA100 GLY101 THR102 PHE103 SER104 ILE105 TYR106 TYR108 A: GLU1062 ARG1063 LEU1064 GLN1065 THR1066 VAL1067 THR1068 ASN1069 ILE1072 ARG1131 SER1137 PRO1138 PHE1139 TYR1141 GLN1142 SER1143 LEU1144 LEU1145 THR1146 PHE1264 LYS1267 GLU1268 ALA1271 LEU1272 THR1274 LEU1275 SER1329 ASP1331 PHE1332 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
