Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1443 |
| PDBID: | 5IWL |
| Chains: | B_C |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | 10.1172/JCI81603 |
| Antibody | |
| Antibody: | 5F9 diabody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Leukocyte surface antigen CD47 |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >5IWL_B|Chain A, B|5F9 diabody|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCKASGYTFTNYNMHWVRQAPGQRLEWMGTIYPGNDDTSYNQKFKDRVTITADTSASTAYMELSSLRSEDTAVYYCARGGYRAMDYWGQGTLVTVSSGGSGGDIVMTQSPLSLPVTPGEPASISCRSSQSIVYSNGNTYLGWYLQKPGQSPQLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDVGVYYCFQGSHVPYTFGQGTKLEIK |
Antigen
Chain: C
Mutation: C15G
| >5IWL_C|Chain C, D|Leukocyte surface antigen CD47|Homo sapiens (9606) QLLFNKTKSVDFTFGNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: TYR27 THR28 ASN31 TYR32 TYR52 GLY54 ASN55 GLY99 GLY100 TYR101 ARG102 ALA103 MET104 ASP105 C: LEU2 LEU3 ASN27 GLU29 GLU100 THR102 ARG103 GLU104 GLY105 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
