Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1441 |
| PDBID: | 5IMO |
| Chains: | B_A |
| Organism: | Camelidae, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.10 |
| Reference: | 10.1016/j.imbio.2016.11.008 |
| Antibody | |
| Antibody: | Nb119 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | mouse Vsig4 in Spacegroup P3221 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >5IMO_B|Chain A[auth B]|Nanobody|Camelidae (9835) QVQLVESGGGLVQAGGSLRLSCAASGRTFSSYGMGWFRQAPGKEREFVAAIRWNGGSTYYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAAGRWDKYGSSFQDEYDYWGQGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >5IMO_A|Chain B[auth A]|Protein Vsig4|Mus musculus (10090) HHHHHHGHPTLKTPESVTGTWKGDVKIQCIYDPLRGYRQVLVKWLVRHGSDSVTIFLRDSTGDHIQQAKYRGRLKVSHKVPGDVSLQINTLQMDDRNHYTCEVTWQTPDGNQVIRDKIIELRVRKYN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLN0 THR27 SER29 SER30 TYR31 ARG51 TRP52 ASN53 ARG99 TRP100 ASP101 LYS102 TYR103 GLY104 PHE107 ASP109 GLU110 ASP112 A: HIS1 THR6 PRO7 GLU8 SER9 LEU38 ARG40 GLY42 SER43 ASP44 SER45 HIS91 THR93 GLU95 ILE107 ARG108 ASP109 LYS110 ILE111 GLU113 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
