Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1440 |
| PDBID: | 5IMM |
| Chains: | B_A |
| Organism: | Camelidae, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 1.20 |
| Reference: | 10.1016/j.imbio.2016.11.008 |
| Antibody | |
| Antibody: | Nb119 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | mouse Vsig4 in Spacegroup P212121 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >5IMM_B|Chain A[auth B]|Nanobody|Camelidae (9835) QVQLVESGGGLVQAGGSLRLSCAASGRTFSSYGMGWFRQAPGKEREFVAAIRWNGGSTYYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAAGRWDKYGSSFQDEYDYWGQGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >5IMM_A|Chain B[auth A]|Protein Vsig4|Mus musculus (10090) HHHHHHGHPTLKTPESVTGTWKGDVKIQCIYDPLRGYRQVLVKWLVRHGSDSVTIFLRDSTGDHIQQAKYRGRLKVSHKVPGDVSLQINTLQMDDRNHYTCEVTWQTPDGNQVIRDKIIELRVRKYN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLN0 THR27 SER29 SER30 TYR31 ARG51 TRP52 ASN53 ARG99 TRP100 ASP101 LYS102 TYR103 GLY104 PHE107 ASP109 GLU110 ASP112 TYR113 A: HIS0 THR5 GLU7 LEU37 ARG39 GLY41 SER42 ASP43 SER44 HIS90 THR92 GLU94 ILE106 ARG107 ASP108 LYS109 ILE110 GLU112 ARG114 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
