Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1436 |
| PDBID: | 5IKC |
| Chains: | BA_M |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.06 |
| Reference: | 10.1074/jbc.M116.726547 |
| Antibody | |
| Antibody: | 6H10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Neuronal migration protein doublecortin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >5IKC_B|Chain B, C[auth H]|Ighg protein|Mus musculus (10090) QVQLQQSGADLVRPGASVKLSCTASGFDIKDDYVHWVKQRPEQGLEWIGRIDPANGATKYAPKFQDKATLTADTSSNTAYLQLSSLTSEDTAVYYCGRSKYFDSWGQGTTLTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEPS |
Light Chain: A
Mutation: NULL
| >5IKC_A|Chain A, D[auth L]|MAb 6H10 light chain|Mus musculus (10090) DIVMTQSQKLMSTSVGDRVSITCKASQIVDTAVAWYQQKPGQSPKPLIYLASNRHTGVPDRFTGSGSGTDFTLTINNVQSDDLADYFCLQHWNYPLTFGAGTKLELKGADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: M
Mutation: NULL
| >5IKC_M|Chain E[auth M], F[auth N]|Neuronal migration protein doublecortin|Homo sapiens (9606) AAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: TYR49 LEU50 ASN53 HIS55 HIS91 TRP92 ASN93 TYR94 LEU96 B: LYS30 ASP31 ASP32 TYR33 ARG50 ASP52 ALA54 ASN55 ARG98 SER99 LYS100 TYR101 ASP103 M: ARG59 ASN60 GLY61 ASP62 ARG63 TYR64 PHE65 LYS66 ASP93 ASN94 ILE95 ASN96 PRO98 ASP130 PHE132 PHE133 LYS134 LYS135 VAL136 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
