Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1416 |
| PDBID: | 5HVG |
| Chains: | B_A |
| Organism: | Homo sapiens, Vicugna pacos |
| Method: | XRD |
| Resolution (Å): | 3.05 |
| Reference: | 10.1111/jth.13381 |
| Antibody | |
| Antibody: | a204 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Carboxypeptidase B2 |
| Antigen mutation: | No |
| Durg Target: | Q96IY4 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >5HVG_B|Chain B, D|VHH-a204|Vicugna pacos (30538) QVQLQESGGGLVQPGGSLRLSCAASGSIFSGNAMGWYRQAPGKQRELVAAITSGGSTDYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCHVDPRPWGYDVTDYDYWGQGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >5HVG_A|Chain A, C|Carboxypeptidase B2|Homo sapiens (9606) FQSGQVLAALPRTSRQVQVLQNLTTTYEIVLWQPVTADLIVKKKQVHFFVNASDVDNVKAHLNVSGIPCSVLLADVEDLIQQQISNDTVSPRASASYYEQYHSLNEIYSWIEFITERHPDMLTKIHIGSSFEKYPLYVLKVSGKEQAAKNAIWIDCGIHAREWISPAFCLWFIGHITQFYGIIGQYTNLLRLVDFYVMPVVNVDGYDYSWKKNRMWRKNRSFYANNHCIGTDLNRNFASKHWCEEGASSSSCSETYCGLYPESEPEVKAVASFLRRNINQIKAYISMHSYSQHIVFPYSYTRSKCKDHEELSLVASEAVRAIEKISKNIRYTYGQGSETLYLAPGGGDDWIYDLGIKYSFTIELRDTGTYGFLLPERYIKPTCREAFAAVSKIAWHVIRNV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: GLN3 SER27 ILE28 SER30 GLY31 ASN32 HIS96 VAL97 PRO101 TRP102 GLY103 TYR104 ASP108 TYR109 ASP110 TYR111 A: PRO91 ALA93 SER94 ALA95 SER96 TYR98 GLU99 ARG117 HIS118 MET121 TRP171 HIS175 THR177 GLN178 PHE179 TYR180 GLY181 ILE182 ILE183 GLY184 GLN185 TYR186 LYS380 ARG384 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
