Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1415 |
| PDBID: | 5HVF |
| Chains: | B_A |
| Organism: | Homo sapiens, Vicugna pacos |
| Method: | XRD |
| Resolution (Å): | 2.85 |
| Reference: | 10.1111/jth.13381 |
| Antibody | |
| Antibody: | i83 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Carboxypeptidase B2 |
| Antigen mutation: | No |
| Durg Target: | Q96IY4 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >5HVF_B|Chain B|VHH-i83|Vicugna pacos (30538) QVQLQESGGGLVQAGGSLRLSCAASGSIFSPNAMGWYRQAPGKERELVAARTNVGSTYADSVKGRFTVSRDNAKNTVYLQMNSLKPEDTAVYYCNAWGQDGWLGQYDYWGQGTQVTVSS |
Antigen
Chain: A
Mutation: NULL
| >5HVF_A|Chain A|Carboxypeptidase B2|Homo sapiens (9606) FQSGQVLAALPRTSRQVQVLQNLTTTYEIVLWQPVTADLIVKKKQVHFFVNASDVDNVKAHLNVSGIPCSVLLADVEDLIQQQISNDTVSPRASASYYEQYHSLNEIYSWIEFITERHPDMLTKIHIGSSFEKYPLYVLKVSGKEQAAKNAIWIDCGIHAREWISPAFCLWFIGHITQFYGIIGQYTNLLRLVDFYVMPVVNVDGYDYSWKKNRMWRKNRSFYANNHCIGTDLNRNFASKHWCEEGASSSSCSETYCGLYPESEPEVKAVASFLRRNINQIKAYISMHSYSQHIVFPYSYTRSKCKDHEELSLVASEAVRAIEKISKNIRYTYGQGSETLYLAPGGGDDWIYDLGIKYSFTIELRDTGTYGFLLPERYIKPTCREAFAAVSKIAWHVIRNV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: ASN32 ALA33 TYR37 GLU44 ARG45 LEU47 ALA50 THR52 ASN53 VAL54 SER56 THR57 TYR58 ASP60 LYS63 ASN95 TRP97 GLY98 GLN99 ASP100 GLY101 TRP102 LEU103 GLY104 GLN105 TYR106 ASP107 TRP109 A: ALA8 ALA9 LEU10 ARG12 THR13 GLN16 GLN33 LYS42 LYS43 GLN45 HIS47 LYS59 ASN63 VAL64 GLY66 ILE67 PRO68 CYS69 SER70 VAL71 LEU72 SER291 GLN292 GLU323 LYS327 ASN328 ILE329 ARG330 ASP366 THR367 GLY368 THR369 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
