Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1411 |
| PDBID: | 5HJ3 |
| Chains: | MN_L |
| Organism: | Ebola virus sp., Ebola virus - Zaire (1995), Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.30 |
| Reference: | 10.1128/mBio.02154-15 |
| Antibody | |
| Antibody: | KZ52 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola Virus GP |
| Antigen mutation: | No |
| Durg Target: | Q05320 |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >5HJ3_M|Chain C[auth A], G[auth E], K[auth I], O[auth M]|KZ52 Antibody Fragment|Homo sapiens (9606) EVQLLESGGGLVKPGGSLRLSCAASGFTLINYRMNWVRQAPGKGLEWVSSISSSSSYIHYADSVKGRFTISRDNAENSLYLQMNSLRAEDTAVYYCVREGPRATGYSMADVFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: N
Mutation: NULL
| >5HJ3_N|Chain D[auth B], H[auth F], L[auth J], P[auth N]|KZ52 Antibody Fragment|Homo sapiens (9606) ELVMTQSPDSLAVSLGERATINCKSSQSVLYSSNNKSYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSAPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLRSPVTKSFNR |
Antigen
Chain: L
Mutation: NULL
| >5HJ3_L|Chain B[auth D], F[auth H], J[auth L], N[auth P]|Envelope glycoprotein|Zaire ebolavirus (128951) EAIVNAQPKCNPNLHYWTTQDEGAAIGLAWIPYFGPAAEGIYTEGLMHNQDGLICGLRQLANETTQALQLFLRATTELRTFSILNRKAIDFLLQRWGGTCHILGPDCCIEPHDWTKNITDKIDQIIHDFVDKTLPD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition M: THR28 ILE30 ASN31 TYR32 SER52 SER53 TYR56 PRO97 ARG98 ALA99 N: ASN28 LYS30 TYR32 TYR49 TRP50 THR53 ARG54 GLU55 SER56 L: ILE504 VAL505 ASN506 ALA507 GLN508 PRO509 LYS510 CYS511 PRO513 ASN514 HIS549 ASN550 GLN551 ASP552 GLY553 LEU554 CYS556 GLN560 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
