Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1403 |
| PDBID: | 5HHV |
| Chains: | HL_A |
| Organism: | Homo sapiens, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 2.20 |
| Reference: | 10.1038/srep26071 |
| Antibody | |
| Antibody: | CAT-2000 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-17A |
| Antigen mutation: | No |
| Durg Target: | Q16552 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5HHV_H|Chain C[auth H]|CAT-2000 FAB heavy chain|Homo sapiens (9606) GSHHHHHHGSENLYFQGEVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARDLIHGVTRNWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPQPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKS |
Light Chain: L
Mutation: NULL
| >5HHV_L|Chain E[auth L]|CAT-2000 FAB light chain|Homo sapiens (9606) NFMLTQPHSVSESPGKTVTISCTRSSGSLANYYVQWYQQRPGSSPTIVIFANNQRPSGVPDRFSGSIDSSSNSASLTISGLKTEDEADYYCQTYDPYSVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTE |
Antigen
Chain: A
Mutation: NULL
| >5HHV_A|Chain A, B|Interleukin-17A|Homo sapiens (9606) DPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYDRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHSPNSFRLEKILVSVGCTCVTPIVHHVA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: ASN-5 LEU-4 SER31 ALA33 SER53 GLY55 TYR58 LEU96 ILE97 HIS98 L: ALA29 ASN30 TYR31 TYR32 PHE49 ALA50 ASN51 GLN53 TYR91 PRO93 A: LYS70 LEU74 TYR85 HIS86 MET87 ASN88 VAL90 PRO91 GLN93 VAL124 THR125 PRO126 ILE127 VAL128 HIS129 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
