Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1400 |
| PDBID: | 5HDQ |
| Chains: | HL_A |
| Organism: | Staphylococcus aureus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 1.83 |
| Reference: | 10.1371/journal.ppat.1005908 |
| Antibody | |
| Antibody: | 305-78-7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | ABC transporter substrate-binding protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5HDQ_H|Chain B[auth H]|Fab Heavy Chain|Mus musculus (10090) QVQLQQSGAELARPGASVKMSCKASGYTFTRYTMHWVQQRPGQGLEWIGYINPSSDYTKYNQKFKDKATMTADKSSSTAYMQLSSLTSEDSAVYYCARKEDVGYWYFDFWGTGTTVTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEPS |
Light Chain: L
Mutation: NULL
| >5HDQ_L|Chain C[auth L]|Fab Light Chain|Mus musculus (10090) DIVMSQSPSSLAVSVGEKVTMSCKSSQSLLYSSNEKNYLAWYQQKPGQSPKLLIYWASTRESGVPDRFTGSGSGTDFTLTISSVKAEDLAVYYCQQYYSYPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >5HDQ_A|Chain A|ABC transporter substrate-binding protein|Staphylococcus aureus (1280) MGTGGKQSSDKSNGKLKVVTTNSILYDMAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKALEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFIDNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGITPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFGEVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR33 TYR50 SER53 SER54 ASP55 TYR56 THR57 LYS58 LYS95 ASP97 VAL98 GLY99 L: TYR32 TYR92 SER93 TYR94 A: LYS233 HIS234 LYS243 GLU247 SER250 GLU251 GLU252 THR253 LYS254 LYS255 ASP256 ILE257 PHE258 GLY259 GLU260 LYS272 THR286 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
