Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1394 |
| PDBID: | 5HBT |
| Chains: | DC_B |
| Organism: | Homo sapiens, Rattus norvegicus, Bungarus multicinctus |
| Method: | XRD |
| Resolution (Å): | 2.61 |
| Reference: | 10.7554/eLife.23043 |
| Antibody | |
| Antibody: | Fab35 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Acetylcholine receptor subunit alpha 1 |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >5HBT_D|Chain C[auth D]|Fab35, Heavy Chain|Rattus norvegicus (10116) EVQLQESGPGLVQPSETLSLTCTVSGFSLTSYSVSWLRQPSGKGPEWMGRMWDDGGTVYNSGLKSRLSISRDTSKNQVFLKMNSLQTDDTGTYYCTRDERIRAINWFAYWGQGTLVTVSSAETTAPSVYPLAPGTALKSNSMVTLGCLVKGYFPEPVTVTWNSGALSSGVHTFPAVLQSGLYTLTSSVTVPSSTWPSQTVTCNVAHPGQQHQRWTRKLC |
Light Chain: C
Mutation: NULL
| >5HBT_C|Chain B[auth C]|Fab35, Light Chain|Rattus norvegicus (10116) DIVITQSPSLLSASVGDRVTLTCKGSQNIDNYLAWYQQKLGEAPKLLIYKTNSLQTGIPSRFSGSGSGTDYTLTISSLHSEDLATYYCYQYINGYTFGTGTKLELKRADAAPTVSIFPPSTEQLATGGASVVCLMNNFYPRDISVKWKIDGTERRDGVLDSVTDQDSKDSTYSMSSTLSLTKADYESHNLYTCEVVHKTSSSPVVKSFNRNEC |
Antigen
Chain: B
Mutation: V9E/W150R/V156A
| >5HBT_B|Chain A[auth B]|Acetylcholine receptor subunit alpha 1|Homo sapiens (9606) KSEHETRLEAKLFKDYSSVVRPVEDHRQVVEVTVGLQLIQLINVDEVNQIVTTNVRLKQQWVDYNLKWNPDDYGGVKKIHIPSEKIWRPDLVLYNNADGDFAIVKFTKVLLQYTGHITWTPPAIFKSYCEIIVTHFPFDEQNCSMKLGTRTYDGSAVAINPESDQPDLSNFMESGEWVIKESRGWKHSVTYSCCPDTPYLDITYHFVMQRLP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ASP1 ASP30 TYR32 LYS50 TYR91 ILE92 ASN93 GLY94 TYR95 D: TRP47 ARG50 TRP52 ASP53 ASP54 GLY56 THR57 VAL58 TYR59 ASN60 LYS64 ARG100 ARG102 ALA103 ILE104 ASN105 B: GLU2 HIS3 ARG6 LEU7 LYS10 LEU11 LYS13 ASP14 TYR15 SER16 GLU23 TYR63 ASN64 LYS66 TRP67 ASN68 PRO69 ASP70 ASP71 TYR72 GLY73 TYR112 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
