Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1393 |
| PDBID: | 5H8O |
| Chains: | A_B |
| Organism: | Lama glama, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 4.21 |
| Reference: | 10.1084/jem.20151790 |
| Antibody | |
| Antibody: | ASC-binding Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Apoptosis-associated speck-like protein containing a CARD |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: A
Mutation: NULL
| >5H8O_A|Chain A|VHH nanobody|Lama glama (9844) QVQLQESGGGLVQPGGSLKLSCAASGFTFSRYAMSWYRQAPGKERESVARISSGGGTIYYADSVKGRFTISREDAKNTVYLQMNSLKPEDTAVYYCYVGGFWGQGTQVTVSSGG |
Antigen
Chain: B
Mutation: NULL
| >5H8O_B|Chain B|Apoptosis-associated speck-like protein containing a CARD|Homo sapiens (9606) IDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ARG52 GLY58 THR59 ILE60 TYR61 TYR62 ASP64 LYS67 B: ALA124 ARG125 GLN185 TYR187 ASP191 ARG194 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
