Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1388 |
| PDBID: | 5H35 |
| Chains: | HI_D |
| Organism: | Mus musculus, Saccharolobus solfataricus |
| Method: | XRD |
| Resolution (Å): | 2.64 |
| Reference: | 10.1038/cr.2016.140 |
| Antibody | |
| Antibody: | 5H35 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Membrane protein TRIC |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5H35_H|Chain A, F, H|Fab Heavy Chain|Mus musculus (10090) EVKLVESGGGLVKPGGSLKLSCAASGFGFTIYDMSWVRQTPEKRLEWVAYMSSGRGNTYYPDTVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYYCTRGAFYYGYGFAYWGQGTLVTVSAAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGPRGPTIKPCPPCKCP |
Light Chain: I
Mutation: NULL
| >5H35_I|Chain B, G, I|Fab Light Chain|Mus musculus (10090) DIVMTQTPLSLPVSLGDQASISCRSSQFIVHSNGNTYLEWYLQKPGQSPKLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYYCFQGSHVPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: D
Mutation: NULL
| >5H35_D|Chain C, D, E|Membrane protein TRIC|Sulfolobus solfataricus (2287) MYMILELLNIIGIIAFTISGSLKGTNKGLDIFGVVTLGVITSYAGGIIADILLGIYPPQILKELNYLLLSVGISIFVFYFYKWLQTNPIKMIIAISDAVGLSTFATLGASLAYSYGLNPISVGLIAAIVGTGGGVIRDVLVNEIPMVLTKEIYATAALLSGFIYYFTTPYLHHDSLFVAFLGSFLLRILSIKYNFNLPLESLESSGENLYFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: VAL2 LYS3 SER25 GLY26 PHE27 GLY28 PHE29 THR30 ILE31 TYR32 SER53 GLY54 ARG55 ASN74 ALA75 ASN77 ARG98 GLY99 ALA100 TYR102 TYR103 TYR105 TYR109 I: TYR54 LYS55 ASN58 PHE60 SER61 D: MET1 TYR2 LEU5 GLU6 ASN9 GLY54 ILE55 TYR56 PRO57 GLN59 ILE60 LYS62 TYR66 LEU107 SER110 LEU111 TYR113 SER114 TYR115 TYR164 THR168 PRO169 HIS172 HIS173 LEU176 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
