Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1376 |
| PDBID: | 5GJS |
| Chains: | HL_B |
| Organism: | Influenza A virus (A/California/04/2009(H1N1)), Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1038/ncomms13577 |
| Antibody | |
| Antibody: | 3E1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H1N1 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5GJS_H|Chain D[auth H]|heavy chain of human neutralizing antibody 3E1|Homo sapiens (9606) QVQLQESGPGLVKPSETLSLTCSVSGASISSYYWIWIRQPAGKGLEWIGRFYTSGSPNYNPSLRSRVTMSVDTSKNQFSLKLTSVTAADTAVYYCAREEHITFGGVIVRYWGQGTLVTVSPASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: L
Mutation: NULL
| >5GJS_L|Chain C[auth L]|light chain of human neutralizing antibody 3E1|Homo sapiens (9606) DIQMTQSPATLSASVGDRVSITCRASQSISSWLAWYQQKPGKAPKLLIYKASSLESGVPSRFSGSGSGSEFTLTISSLQPDDFAIYYCQQYNSYPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >5GJS_B|Chain B|Hemagglutinin|Influenza A virus (641501) GLFGAIAGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDEITNKVNSVIEKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENERTLDYHDSNVKNLYEKVRSQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGTYDYPKYSEEAKLNREEIDGVLELVPR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: SER30 SER31 TRP32 LYS50 TYR91 ASN92 SER93 TYR94 H: SER31 TYR32 TYR33 ARG50 TYR52 SER54 GLY55 SER56 GLU98 GLU99 HIS100 ILE101 THR102 PHE103 GLY105 VAL106 B: THR15 GLY16 MET17 VAL18 ASP19 GLY20 TRP21 HIS26 SER32 GLY33 TYR34 ALA35 LEU38 THR41 GLN42 ILE45 ASP46 ILE48 THR49 VAL52 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
