Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1361 |
| PDBID: | 5FYK |
| Chains: | DE_B |
| Organism: | Human immunodeficiency virus 1, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.11 |
| Reference: | 10.1016/j.cell.2016.04.010 |
| Antibody | |
| Antibody: | 35O22 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | JR-FL GP41 ENV ECTODOMAIN |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >5FYK_D|Chain B[auth D]|35O22|HOMO SAPIENS (9606) QGQLVQSGAELKKPGASVKISCKTSGYRFNFYHINWIRQTAGRGPEWMGWISPYSGDKNLAPAFQDRVIMTTDTEVPVTSFTSTGAAYMEIRNLKFDDTGTYFCAKGLLRDGSSTWLPYLWGQGTLLTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKGLEVLFQ |
Light Chain: E
Mutation: NULL
| >5FYK_E|Chain C[auth E]|35O22|HOMO SAPIENS (9606) QSVLTQSASVSGSLGQSVTISCTGPNSVCCSHKSISWYQWPPGRAPTLIIYEDNERAPGISPRFSGYKSYWSAYLTISDLRPEDETTYYCCSYTHNSGCVFGTGTKVSVLGQSKANPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: B
Mutation: NULL
| >5FYK_B|Chain A[auth B]|JR-FL, GP41 ENV ECTODOMAIN|HUMAN IMMUNODEFICIENCY VIRUS 1 (11676) AVGIGAVFLGFLGAAGSTMGAASMTLTVQARLLLSGIVQQQNNLLRAPEAQERMLQLTVWGIKQLQARVLAVERYLGDQQLLGIWGCSGKLICCTAVPWNASWSNKSLDRIWNNMTWMEWEREIDNYTSEIYTLIEESQNQQEKNEQELLELDGGLEVLFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition D: PHE31 TYR32 PHE72 LEU96 LEU97 ARG98 ASP99 E: TYR49 GLU50 GLU53 ARG54 PRO56 B: GLY527 SER528 THR529 ALA532 SER618 ASP620 ARG621 ASN624 ASN625 THR627 MET629 GLU630 ARG633 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
