Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1351 |
| PDBID: | 5FUC |
| Chains: | V_AD |
| Organism: | Homo sapiens, Camelus dromedarius |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1038/srep37716 |
| Antibody | |
| Antibody: | VHH6 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | IL-6 and gp80 complex |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: V
Mutation: NULL
| >5FUC_V|Chain E, F[auth V]|VHH6|CAMELUS DROMEDARIUS (9838) DVQFVESGGGSVHAGGSLRLNCATSGYIYSTYCMGWFRQAPGKEREGVAHIYTNSGRTYYADSVKGRFTISQDNAKNTVYLQMNSLKPEDTAIYYCAARPSIRCASFSATEYKDWGQGTQVTVSSRENLYFQ |
Antigen
Chain: AD
Mutation: NULL
| >5FUC_A|Chain A, B|INTERLEUKIN-6|HOMO SAPIENS (9606) GSSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM >5FUC_D|Chain C, D|INTERLEUKIN-6 RECEPTOR SUBUNIT ALPHA, INTERLEUKIN-6 RECEPTOR|HOMO SAPIENS (9606) GLAPRRCPAQEVARGAGAGDVPPEEPQLSCFRKSPLSNVVCEWGPRSTPSLTTKAVLLVRKFQNSPAEDFQEPCQYSQESQKFSCQLAVPEGDSSFYIVSMCVASSVGSKFSKTQTFQGAGILQPDPPANITVTAVARNPRWLSVTWQDPHSWNSSFYRLRFELRYRAERSKTFTTWMVKDLQHHAVIHDAWSGLRHVVQLRAQEEFGQGEWSEWSPEAMGTPWTESRSPP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition V: VAL2 GLN3 SER25 GLY26 TYR27 ILE28 TYR29 SER30 THR31 TYR32 TYR52 THR53 ASN54 SER55 GLY56 ARG57 GLN72 ASN74 ALA75 ASN77 PRO100 SER101 ILE102 ARG103 LYS113 A: GLY19 SER20 SER22 GLU23 ASP26 GLN75 PHE78 GLU80 ARG182 GLN183 MET184 D: SER216 THR218 TRP219 GLN220 ASP221 HIS223 ASN226 SER227 SER228 PHE229 ARG231 LEU254 GLN255 HIS256 HIS257 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
