Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1348 |
| PDBID: | 5FHC |
| Chains: | HL_K |
| Organism: | Ebola virus - Mayinga, Zaire, 1976, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 6.70 |
| Reference: | 10.1126/science.aad6117 |
| Antibody | |
| Antibody: | 114 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Ebola Virus Fusion Glycoprotein (GP) |
| Antigen mutation: | No |
| Durg Target: | Q05320 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5FHC_H|Chain F[auth H]|Antibody 114 Fab heavy chain|Homo sapiens (9606) EVQLVESGGGLIQPGGSLRLSCAASGFALRMYDMHWVRQTIDKRLEWVSAVGPSGDTYYADSVKGRFAVSRENAKNSLSLQMNSLTAGDTAIYYCVRSDRGVAGLFDSWGQGILVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: L
Mutation: NULL
| >5FHC_L|Chain E[auth L]|Antibody 114 Fab light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRITITCRASQAFDNYVAWYQQRPGKVPKLLISAASALHAGVPSRFSGSGSGTHFTLTISSLQPEDVATYYCQNYNSAPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG |
Antigen
Chain: K
Mutation: NULL
| >5FHC_K|Chain B[auth K]|Envelope glycoprotein,Envelope glycoprotein|Zaire ebolavirus (128952) MGVTGILQLPRDRFKRTSFFLWVIILFQRTFSIPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWKVNPEIDTTIGEWAFWETKKNLTRKIRSEELSFSRAGLITGGRRTRR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition L: TYR32 ALA53 LEU54 HIS55 ALA56 GLY57 ASN92 SER93 ALA94 LEU96 H: GLY26 PHE27 ARG30 MET31 TYR32 ASP33 HIS35 TRP47 ALA50 GLY52 PRO53 SER54 ASP56 TYR58 ASP96 ARG97 GLY98 VAL99 K: GLU112 LYS114 LYS115 PRO116 ASP117 GLY118 SER119 GLU120 SER142 GLY143 THR144 GLY145 PRO146 CYS147 ARG172 GLN221 ALA222 THR223 GLY224 THR227 GLU229 GLU231 LEU233 ASN238 LEU239 TYR241 THR269 SER309 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
