Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 133 |
| PDBID: | 1OTU |
| Chains: | CD_A |
| Organism: | Escherichia coli, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.30 |
| Reference: | 10.1126/science.1082708 |
| Antibody | |
| Antibody: | 1OTU Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Voltage-gated ClC-type chloride channel eriC |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >1OTU_C|Chain C, E|Fab fragment (Heavy chain)|Mus musculus (10090) EVRLLESGGGLVQPGGSLKLSCAASGFDYSRYWMSWVRQAPGKGLKWIGEINPVSSTINYTPSLKDKFIISRDNAKDTLYLQISKVRSEDTALYYCARLYYGYGYWYFDVWGAGTTVTVSSAKTTPPSVYPLAPGSAAAAASMVTLGCLVKGYFPEPVTVTWNSGSLAAGVHTFPAVLQAALYTLSSSVTVPSSSWPSETVTCNVAHPASSTKVDKKIVPRA |
Light Chain: D
Mutation: NULL
| >1OTU_D|Chain D, F|Fab fragment (Light chain)|Mus musculus (10090) DIVLTQSPAIMSAAPGDKVTMTCSASSSVSYIHWYQQKSGTSPKRWIYDTSKLTSGVPVRFSGSGSGTSYSLTINTMEAEDAATYYCQQWSSHPQTFGGGTKLEILRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRA |
Antigen
Chain: A
Mutation: E148Q
| >1OTU_A|Chain A, B|Voltage-gated ClC-type chloride channel eriC|Escherichia coli (562) MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGTLVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAPEAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGRQGPTVQIGGNIGRMVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAVFIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGMQDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLVFIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIAGMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILARTLAKQEAEQLARSK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ARG31 TRP33 GLU50 ASN52 PRO53 VAL54 SER56 THR57 ASN59 TYR100 TYR101 GLY102 TYR103 GLY104 TYR105 TRP106 D: TRP90 SER91 SER92 HIS93 A: LYS243 LEU244 SER245 ASP246 ALA247 PRO248 LEU249 ASN250 VAL376 PRO380 GLN381 TYR382 HIS383 LEU384 GLU385 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
