Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1309 |
| PDBID: | 5F6J |
| Chains: | BA_G |
| Organism: | Human immunodeficiency virus 1, Macaca mulatta |
| Method: | XRD |
| Resolution (Å): | 6.63 |
| Reference: | 10.1016/j.celrep.2015.12.017 |
| Antibody | |
| Antibody: | DH427 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 clade C gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >5F6J_B|Chain D[auth B], F[auth H]|DH427 Antibody Heavy Chain|Macaca mulatta (9544) EVQLVESGGGLVQPGGSLRLSCAASGFTFSNSGMIWVRQAPGKGLEWVSYISLSGANTYYADSVKGRFTISRDNSQNTLSLQMNSLRVEDTAMYYCAKEGWSYFDFWGQGVLVTVSGASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKHHHHHH |
Light Chain: A
Mutation: NULL
| >5F6J_A|Chain C[auth A], E[auth F]|DH427 Antibody Light Chain|Macaca mulatta (9544) QSALTQPPSVSKSLGQSVTISCTGTSSDIGAYTGVSWYQQHSGTAPRLLIYDVSKRPSGVSDRFSGSKSGNTASLTISGLQTDDEADYYCCSYRTGATYIFGTGTRVTVLGQPKGAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: G
Mutation: NULL
| >5F6J_G|Chain A[auth E], B[auth G]|ENVELOPE GLYCOPROTEIN GP120 of HIV-1 clade C|Human immunodeficiency virus 1 (11676) VWKEAKTTLFCASDAKAYEREVHNVWATHACVPTDPNPQEMALGNVTENFNMWKNDMVDQMHEDIISLWDQSLKPCVKLTGGSTVTQACPKVSFDPIPIHYCAPAGYAILKCNNETFEGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAKEEIVIRSENLTDNAKTIIVHLKESVEIVCTRPGNGGSGSGGDTRQAHCNISEEKWNKTLQKVSKILQEHFPNKAIKFEPHSGGDLEITTHSFNCRGEFFYCNTTKLFNGTYNSTANSTGSVSNTTITLPCRIKQIVNMWQGVGRAMYAPPIKGNITCKSIITGLLLTRDKDSNTETFRPGGGDMRDNWRSELYKYKVVEIK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: ALA31 TYR32 THR33 GLY34 ASP52 LYS55 TYR93 THR95 GLY96 ALA97 TYR99 B: TYR50 SER52 LEU53 SER54 ALA56 ASN57 TYR59 GLU99 TRP101 SER102 G: GLN350 PHE353 PRO354 ASN355 LYS357 ALA358 LYS360 GLU362 THR394 ARG456 ASP457 LYS458 ASP462 SER463 ASN464 THR465 GLU466 THR467 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
