Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1306 |
| PDBID: | 5F3H |
| Chains: | CD_I |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1080/19420862.2016.1215786 |
| Antibody | |
| Antibody: | RK35 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Growth/differentiation factor 8 |
| Antigen mutation: | No |
| Durg Target: | O14793 |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >5F3H_C|Chain A, C, E, G|humanized RK35 antibody heavy chain|Mus musculus (10090) EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSTISSGGSYTSYPDSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKQDYAMNYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: D
Mutation: NULL
| >5F3H_D|Chain B, D, F, H|humanized RK35 antibody light chain|Mus musculus (10090) DIQMTQSPSSLSASVGDRVTITCKASQDVSTAVAWYQQKPGKAPKLLIYSASYRYTGVPSRFSGSGSGTDFTLTISSLNPEDFATYYCQQHYSTPWTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: I
Mutation: NULL
| >5F3H_I|Chain I, J, K, L|Growth/differentiation factor 8|Homo sapiens (9606) FGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFVFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIPAMVVDRCGCS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: SER31 TYR32 ALA33 THR50 SER52 SER53 GLY54 SER56 TYR57 THR58 SER59 TYR60 GLN99 ASP100 TYR101 D: SER30 THR31 ALA32 SER50 TYR53 HIS91 TYR92 SER93 THR94 PRO95 TRP96 I: GLU25 ASP30 TRP31 ILE33 ALA34 PRO35 LYS36 ASN83 LEU85 PHE87 ASN88 GLY89 GLU91 GLN92 ILE93 ILE94 TYR95 LYS97 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
