Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1302 |
| PDBID: | 5F21 |
| Chains: | B_A |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 1.90 |
| Reference: | 10.1038/srep27055 |
| Antibody | |
| Antibody: | MU375 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1(CD38) |
| Antigen mutation: | No |
| Durg Target: | P28907 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >5F21_B|Chain B|nanobody MU375|Lama glama (9844) DVQLQESGGGSVQAGGSLTLSCTASGLLFRLASMGWYRQAPGKERELIATITVGGKTNYKDSVQGRFIITRDNTGDNTKSTVTLQMNRLKPEDTAVYYCNTASPAVGADTWGQGTRVTVSSEPKTPKPQPAAAHHHHHHGAAEQKLISEEDLNGAA |
Antigen
Chain: A
Mutation: NULL
| >5F21_A|Chain A|ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1|Homo sapiens (9606) WRQTWSGPGTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGAFISKHPCDITEEDYQPLMKLGTQTVPCNKILLWSRIKDLAHQFTQVQRDMFTLEDTLLGYLADDLTWCGEFDTSKINYQSCPDWRKDCSNNPVSVFWKTVSRRFAEAACDVVHVMLDGSRSKIFDKDSTFGSVEVHNLQPEKVQTLEAWVIHGGREDSRDLCQDPTIKELESIISKRNIQFSCKNIYRPDKFLQCVKNPEDSSCTSEI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: GLN3 GLY26 LEU27 LEU28 PHE29 LEU31 SER33 LEU47 ILE48 ALA49 THR50 THR52 VAL53 GLY54 LYS56 ASN58 TYR59 LYS60 THR78 ALA102 SER103 PRO104 ALA105 VAL106 GLY107 ASP109 THR110 A: LYS121 PHE135 GLN139 MET142 HIS205 GLU239 TRP241 ASP252 CYS254 SER274 CYS275 LYS276 ASN277 ILE278 TYR279 ARG280 LYS283 PHE284 CYS287 VAL288 PRO291 GLU292 ASP293 SER294 SER295 CYS296 THR297 SER298 GLU299 ILE300 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
