Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1298 |
| PDBID: | 5EZO |
| Chains: | HL_A |
| Organism: | Homo sapiens, Mus musculus, Plasmodium falciparum 3D7 |
| Method: | XRD |
| Resolution (Å): | 3.63 |
| Reference: | 10.7554/eLife.20383 |
| Antibody | |
| Antibody: | c12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | PfCyRPA |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5EZO_H|Chain A[auth H]|PfCyRPA|Homo sapiens (9606) NVNLLESGGGLVQPGGSLNLSCAASGFDFSRYWMSWARQAPGKGQEWIGEINPGSSTIKYTPSLKDKFIISRDNAKNTLYLQMSKVSSEDTALYYCARYGSYVYAMDYWGPGTSVTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGPT |
Light Chain: L
Mutation: NULL
| >5EZO_L|Chain B[auth L]|c12 FAB|Mus musculus (10090) SIVMTQTPKFLLVSAGDRITITCKASQSVRNDVAWYQQKPGQSPKLLIYFASNRYTGVPDRFTGSGSGTDFTFTISTVQAEDLAVYFCQQGYTSPRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: A
Mutation: NULL
| >5EZO_A|Chain C[auth A]|c12 Fab|Plasmodium falciparum (isolate 3D7) (36329) RHVFIRTELSFIKNNVPCIRDMFFIYKRELYNICLDDLKGEEDETHIYVQKKVKDSWITLNDLFKETDLTGRPHIFAYVDVEEIIILLCEDEEFSNRKKDMTCHRFYSNDGKEYNNSEITISDYILKDKLLSSYVSLPLKIENREYFLICGVSPYKFKDDNKKDDILCMASHDKGETWGTKIVIKYDNYKLGVQYFFLRPYISKNDLSFHFYVGDNINNVKNVNFIECTHEKDLEFVCSNRDFLKDNKVLQDVSTLNDEYIVSYGNDNNFAECYIFFNNENSILIKPEKYGNTTAGCYGGTFVKIDENRTLFIYSSSQGIYNIHTIYYANYE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: ARG49 TRP51 ASN70 SER74 LYS77 TYR117 GLY118 SER119 TYR120 VAL121 TYR122 L: SER48 ARG50 ASN51 ASP52 TYR69 PHE70 SER72 ASN73 GLY86 SER87 GLY88 TYR112 THR113 ARG116 A: LYS43 ASN45 VAL46 PRO47 ASP66 LEU68 ASP73 GLU74 HIS76 TYR78 GLN80 TRP87 ILE88 THR89 LEU90 ASN91 ASP92 LEU93 LYS95 GLU96 THR97 ASP98 PHE136 GLU143 TYR144 ASN146 SER147 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
