Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1290 |
| PDBID: | 5EN2 |
| Chains: | AB_C |
| Organism: | Mus musculus, Mammarenavirus juninense |
| Method: | XRD |
| Resolution (Å): | 1.82 |
| Reference: | 10.1016/j.chom.2015.11.005 |
| Antibody | |
| Antibody: | GD01 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Pre-glycoprotein polyprotein GP complex |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >5EN2_A|Chain A|GD01 heavy chain|Mus musculus (10090) EVKLQQSGAELARPGTSVKLSCKASGYTFTNYWMQWIKQRPGQGLEWIGAVYPGDGDTRFSQKFKGKATLTADKSSSTAYMQLSSLSSEDSAVYFCARRRVYYGSNYIYALDYWGQGTSVTVSAAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPR |
Light Chain: B
Mutation: NULL
| >5EN2_B|Chain B|GD01 light chain|Mus musculus (10090) DIVMTQSQKFMSTSIGDRVSITCKASQNVGSAVAWYQQKPGQSPKLLIYSASNRYTGVPDRFIGSESGTDFTLTISNMQSEDLADYFCQQYSSYPLAFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN |
Antigen
Chain: C
Mutation: NULL
| >5EN2_C|Chain C|Pre-glycoprotein polyprotein GP complex|Junin mammarenavirus (2169991) DLPLLCTLNKSHLYIKGGNASFKISFDDIAVLLPEYDVIIQHPADMSWCSKSDDQIWLSQWFMNAVGHDWYLDPPFLCRNRTKTEGFIFQVNTSKTGINENYAKKFKTGMHHLYREYPDSCLDGKLCLMKAQPTSWPLQCP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: TRP33 GLY53 ASP54 ASP56 THR57 ARG58 PHE59 GLN61 LYS64 ARG95 VAL97 TYR98 TYR99 B: ASP1 ILE2 ASN28 VAL29 GLY30 SER31 ALA32 GLU66 TYR91 SER92 SER93 TYR94 PRO95 C: HIS98 LYS109 SER111 PHE112 ASP113 ASP114 ILE115 ALA116 VAL117 LEU119 GLU121 TYR122 ASP123 ARG165 THR168 LYS169 THR170 GLU171 THR182 GLY210 LYS211 LEU212 LYS216 GLN218 PRO219 THR220 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
