Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1283 |
| PDBID: | 5E94 |
| Chains: | BA_G |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.00 |
| Reference: | 10.1038/srep26236 |
| Antibody | |
| Antibody: | 3F52 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Glucagon-like Peptide-1 receptor extracellular domain |
| Antigen mutation: | No |
| Durg Target: | P43220 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >5E94_B|Chain B, D|Antibody Fab fragment heavy chain|Mus musculus (10090) DVQLQESGPGLVKPSQSLSLTCTVTGYSITSTYDWHWIRHFPGNILEWMGYISYSGSTNYNPSLKSRISITHDTSKNRFFLKLNSVTSEDTATYYCARATASFYDGSYYFDYWGQGTTLTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESK |
Light Chain: A
Mutation: NULL
| >5E94_A|Chain A, C|Antibody Fab fragment light chain|Mus musculus (10090) DIQMTQTTSSLSASLGDRVTISCRASQDISNYLNWYQQKPDGTVKLLIYYTSRLHSGVPSRFSGSGSGTDYSLTISSLEQEDVATYFCQQGNTLPFTFGSGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: G
Mutation: NULL
| >5E94_G|Chain E[auth G], F[auth H]|Glucagon-like peptide 1 receptor|Homo sapiens (9606) GSHMRPQGATVSLWETVQKWREYRRQCQRSLTEDPPPATDLFCNRTFDEYACWPDGEPGSFVNVSCPWYLPWASSVPQGHVYRFCTAEGLWLQKDNSSLPWRDLSECEESKRGERSSPEEQLLFLY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: TYR32 TYR50 ARG53 GLY91 ASN92 THR93 LEU94 PHE96 B: TYR27 SER31 THR32 TYR33 ASP34 TYR51 SER53 TYR54 SER55 SER57 THR58 ALA99 THR100 ALA101 SER102 PHE103 TYR104 ASP105 GLY106 TYR108 G: THR29 VAL30 SER31 LEU32 TRP33 THR35 VAL36 GLN37 TRP39 ARG40 ARG43 GLU68 PRO90 PRO137 GLU138 LEU141 LEU142 LEU144 TYR145 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
