Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1282 |
| PDBID: | 5E8E |
| Chains: | BA_H |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.90 |
| Reference: | 10.1111/jth.13171 |
| Antibody | |
| Antibody: | 5E8E Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | THROMBIN |
| Antigen mutation: | No |
| Durg Target: | P00734 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >5E8E_B|Chain B|IGA FAB HEAVY CHAIN|Homo sapiens (9606) QVQLIQSGSAVKKPGASVRVSCKVSGYTLTEAAIHWVRQAPGKGLEWMGGLDPQDGETVYAQQFKGRVTMTEDRSTDTAYMEVNNLRSEDTATYYCTTGDFSEFEPFSMDYFHFWGQGTVVTVASSTPVSPKVFPLSLCSTQPDGNVVIACLVQGFFPQEPLSVTWSESGQGVTARNFPPSQDASGDLYTTSSQLTLPATQCLAGKSVTCHVKHYTNPSQDVTVPCPVP |
Light Chain: A
Mutation: NULL
| >5E8E_A|Chain A|IGA FAB LIGHT CHAIN|Homo sapiens (9606) EIVLTQSPATLSLSPGERATLSCRASQNVSSFLAWYQHKPGQAPRLLIYDASSRATDIPIRFSGSGSGTDFTLTISGLEPEDFAVYYCQQRRSWPPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKQKVYACEVTHQGLSSPVTKSFLRGEC |
Antigen
Chain: H
Mutation: NULL
| >5E8E_H|Chain C[auth H]|THROMBIN HEAVY CHAIN|Homo sapiens (9606) IVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQFGE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: TYR49 B: THR30 PRO53 GLN54 ASP55 GLY56 ASP100 SER102 GLU103 PHE104 GLU105 PRO106 PHE107 SER108 H: PHE339 SER342 PRO343 GLN344 GLU345 LEU346 LEU380 ARG382 ARG388 THR389 ARG390 TYR391 ARG393 ILE398 GLN476 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
