Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1281 |
| PDBID: | 5E8D |
| Chains: | HL_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1074/jbc.M115.656009 |
| Antibody | |
| Antibody: | 9E5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Proepiregulin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >5E8D_H|Chain B[auth H]|anti-human epiregulin antibody 9E5 Fab heavy chain|Mus musculus (10090) EVQLQQSGAELVKPGASVKLSCTASGFNIKDTYMHWVKQRPEQGLEWIGRIDPLNDKTKYDPKFQGKATITADTSSNSAYLQLSSLTSEDTAVYYCSRGGGDPVFVYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: L
Mutation: NULL
| >5E8D_L|Chain C[auth L]|anti-human epiregulin antibody 9E5 Fab light chain|Mus musculus (10090) DIQMTQSPSSLSASLGGKVTITCKASQDINKYIAWYQHKPGKGPRLLIHYTSTLHPGIPSRFSGSGSGRDYSFSISNLEPEDIATYYCLQYDNLRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >5E8D_A|Chain A|Proepiregulin|Homo sapiens (9606) GPAMSCIPGESSDNCTALVQTEDNPRVAQVSITKCSSDMNGYCLHGQCIYLVDMSQNYCRCEVGYTGVRCEHFFL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: LYS30 ASP31 THR32 TYR33 ARG50 ASP52 LEU54 ARG98 GLY100 GLY101 ASP102 PRO103 VAL104 VAL106 L: ASN30 TYR32 LEU46 HIS49 TYR50 THR53 LEU54 HIS55 PRO56 TYR91 ASP92 ASN93 LEU94 ARG95 A: THR4 LYS5 SER7 SER8 ASP9 MET10 ASN11 GLY12 TYR13 LEU15 TYR21 VAL23 ASP24 MET25 SER26 GLN27 ASN28 VAL39 ARG40 GLU42 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
