Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 128 |
| PDBID: | 1OSP |
| Chains: | HL_O |
| Organism: | Mus musculus, Borreliella burgdorferi B31 |
| Method: | XRD |
| Resolution (Å): | 1.95 |
| Reference: | 10.1073/pnas.94.8.3584 |
| Antibody | |
| Antibody: | 184.1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | OUTER SURFACE PROTEIN A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >1OSP_H|Chain B[auth H]|FAB 184.1|Mus musculus (10090) EVQLQESGPSLVKPSQTLSLTCSVTGEPITSGFWDWIRKFPGNKLEFMGYIRYGGGTYYNPSLKSPISITRDTSKNHYYLQLNSVVTEDTATYYCARSRDYYGSSGFAFWGEGTLVTVSAAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLE |
Light Chain: L
Mutation: NULL
| >1OSP_L|Chain A[auth L]|FAB 184.1|Mus musculus (10090) DIQMSQSSSSFSVSLGDRVTITCKASEDIYSRLAWYQQKPGNAPRLLISGATSLETWVPSRFSGSDSGKDYTLSITSLQTEDVATYFCQQYWSPPPTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: O
Mutation: NULL
| >1OSP_O|Chain C[auth O]|OUTER SURFACE PROTEIN A|Borrelia burgdorferi (224326) AKQNVSSLDEKNSVSVDLPGEMKVLVSKEKNKDGKYDLIATVDKLELKGTSDKNNGSGVLEGVKADKCKVKLTISDDLGQTTLEVFKEDGKTLVSKKVTSKDKSSTEEKFNEKGEVSEKIITRADGTRLEYTGIKSDGSGKAKEVLKGYVLEGTLTAEKTTLVVKEGTVTLSKNISKSGEVSVELNDTDSSAATKKTAAWNSGTSTLTITVNSKKTKDLVFTKENTITVQQYDSNGTKLEGSAVEITKLDEIKNALK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR30 SER31 ARG32 SER49 GLY50 THR52 SER53 ASP66 TYR91 TRP92 H: GLU27 SER31 TYR53 ARG97 ARG99 ASP100 TYR101 TYR102 GLY103 SER104 O: VAL30 VAL32 ASP33 LEU34 PRO35 VAL42 SER43 LYS44 GLU45 LYS46 TYR52 ASP68 LYS69 ASN70 ASN71 ASP92 ASP93 LEU94 GLY95 LYS117 ASP118 LYS119 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
