Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 1279 |
| PDBID: | 5E5M |
| Chains: | F_E |
| Organism: | Mus musculus, Camelidae |
| Method: | XRD |
| Resolution (Å): | 2.18 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | CTLA-4 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Cytotoxic T-lymphocyte protein 4 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: F
Mutation: NULL
| >5E5M_F|Chain B, D, F, H|CTLA-4 nanobody|Camelidae (9835) MAQVQLVESGGGLAQPGGSLRLSCAASGSTISSVAVGWYRQTPGNQREWVATSSTSSTTATYADSVKGRFTISRDNAKNTIYLQMNSLKPEDTAVYYCKTGLTNWGRGTQVTVSSGGLPETGGHHHHHH |
Antigen
Chain: E
Mutation: NULL
| >5E5M_E|Chain A, C, E, G|Cytotoxic T-lymphocyte protein 4|Mus musculus (10090) IQVTQPSVVLASSHGVASFPCEYSPSHNTDEVRVTVLRQTNDQMTEVCATTFTEKNTVGFLDYPFCSGTFNESRVNLTIQGLRAVDTGLYLCKVELMYPPPYFVGMGNGTQIYVIDP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: VAL4 SER33 VAL34 ALA35 GLY37 TYR39 GLN46 ARG47 TRP49 THR52 SER53 THR55 SER57 THR58 THR59 THR61 LYS99 THR100 GLY101 LEU102 THR103 TRP105 E: TYR23 PRO25 SER26 HIS27 ASN28 THR29 ASP30 VAL32 PHE52 THR53 GLU54 LYS55 THR57 PHE70 GLU72 SER73 TYR98 PRO99 PRO100 PRO101 TYR102 PHE103 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
